Superdex 200 increase from, your source for laboratory equipment and scientific supplies. Jun 12, 2017 superdex 30 increase is part of ges new generation of agarosebased sec columns called increase, which offer higher resolution purification in shorter runtimes compared with their predecessors. Superdex prep grade and prepacked hiload columns 1 chromatography media characteristics 2 chemical stability and dextran. Superdex 75 10300 gl is a prepacked gel filtration column for highresolution, semipreparative, and analytical separations of biomolecules.
Superdex 75 has a separation range for molecules with molecular weights between 3 000 and 70 000. Superdex 75 increase 5150 gl is for rapid size analysis of proteins and other biomolecules. Superdex 75 increase 5150 gl an d superdex 75 increase 10300 gl columns are intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes. The maximum column pressure for the superdex 200 column is 218 psi, and the recommended flow rate is 0. So if people are dong a manual clean it can easily happen that system will go over pressure limit of. Rapid purity check and screening superdex 75 5150 gl, superdex 200 increase 5150 gl and superose 6 increase 5150 gl are short columns with small bed volumes that are suitable for rapid protein homogeneity analyses or purity checks. For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Pdf legumain expression, activity and secretion are. Buffers and solvents with increased viscosity will affect the backpressure and flow rate. Superdex 30, 75 and 200 prep grade are preparative gel filtration media with a unique. Superdex 75 10300 gl and superdex 200 10300 gl are tricorn high performance columns. Ge healthcare size exclusion chromatography superdex 30 increase columns. Ge healthcare hiload 16600 superdex 200 pg hiload 1660.
Hiload 16600 superdex 75 pg cytiva formerly ge healthcare. This material is provided for educational purposes only and is not intended for medical advice, diagnosis or treatment. Size exclusion chromatography columns and media fisher scientific. Superdex 75 5150 gl and superdex 200 5150 gl are short columns with small bed volumes that are suitable for rapid protein homogeneity analyses or purity checks. Highresolution analysis and purification of small sample volumes, especially when sample amount is limited and low buffer consumption is important. Instructions 56119099 ae gel filtration media superdex. The low molecular weight kit contains 5 proteins with molecular weights in the range 6500 to 75 000 and blue dextran 2000. Chemical, physical and chromatographic properties of superdex. The columns are prepacked glass columns for high performance gel filtration of proteins, peptides, dna fragments 48,000. Superdex 30 increase ges newest sec column lab equipment.
Superdex 200 increase smallscale sec columns cytiva. Superdex 75 has a separation range for molecules with molecular weights between 3,000 and 70,000. Ge healthcare size exclusion chromatography superdex 30. General description superdex 75 10300 gl is a prepacked gel filtration column for highresolution, semipreparative, and analytical separations of biomolecules. New superdex 200 increase columns fisher scientific. Data file 29045269aa size exclusion chromatography superdex 200 increase columns superdex 200 increase prepacked columns fig 1 are designed for size exclusion chromatography sechigh resolution gel filtration in smallscale g to mg, preparative purification, as well as for characterization and analysis. Use this column for rapid purity check and screening of proteins with molecular weights between mr 3,000 and 70,000, such as recombinant tagged proteins. Hiload superdex 30, 75, and 200 prep grade columns bring convenience and high resolution to gel filtration. Dextran component determined the separation properties of these media. Hiload superdex 75 prep grade data file hiload superdex. Sec columns for analysis and purification of peptides superdex 30 increase is suitable for both preparative and analytical purposes. Superdex 200 increase size exclusion chromatography columns are well suited for highresolution analysis and smallscale purification of monoclonal antibodies mab and other biomolecules with m r 10 000 to 600 000.
For olecules with molecular weights between 0 and 600000 da. Superdex 30, 75, and 200 prep grade media are available prepacked in hiload 1660 and hiload 2660 columns. Ge healthcare superdex 200 increase 10300 gl prepacked tricorn columns for size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Hiload superdex 75 prep grade separates proteins and peptides in the molecular weight range 3 00070 000 daltons and performs best between 8 000 and 50 000, which is ideal for recombinant proteins. Is it possible to rescue, to some degree, a compressed gel filtration column superdex 75 or 200 1030 gl. Flow rate limits and recommendations superdex 75 increase 10300 gl note. Most waterbased buffers can be considered to be similar to water, for example phosphate and tris buffers. Superdex 75 increase 10300 gl is suitable for both smallscale preparative purification and for analytical applications. Instruction 29163059 ae size exclusion chromatography columns. Introduction two gel filtration calibration kits are available for protein molecular weight determination by gel filtration.
Superdex 75 10300 gl from ge healthcare selectscience. Superdex prep grade pg is produced by covalent bonding of dextran to highly crosslinked agarose. Superdex 200 increase prepacked columns deliver up to 50% higher resolution in less time than the predecessors superdex 200. With their high physical and chemical stability, very high batchtobatch reproducibility, and regulatory support file backup, superdex 30 prep grade, superdex 75 prep grade and superdex 200 prep grade are ideal for all stages of an industrial scale operation from research and. The columns are prepacked glass columns for high performance gel filtration of proteins, peptides, dna fragments superdex 75 increase is designed for smallscale preparative purification and analysis of recombinant tagged or other proteins with molecular weights from 3000 up to 70 000, such as recombinant tagged proteins features and benefits comprising smaller, more rigid beads. As shown in figures 4 a and b, the detection signal from the run with superdex 200 increase 3. Hiload superdex 30 prep grade hiload superdex 75 prep grade hiload superdex 200 prep grade hiload columns are xk laboratory columns prepacked with superdex prep grade for gel filtration. Promo code superdex 200 increase 10300 gl suitable for both smallscale mg, preparative and highresolution analysis applications 28990944 45002570 45002570pr superdex 200 increase 5150 gl designed for rapid purity checking and quick screening of different. Superdex 75 increase cytiva, formerly ge healthcare life sciences. Ge healthcare superdex 75 increase prepacked column, 5150 gl. Igg pdf structural characterization of vapb46 antitoxin from. Data file 18116379 ad gel filtration superdex high.
Instructions 56119099 ae gel filtration media superdex 30. Protein purification chromatography calibration of hiload. Start with superdex 200 increase when the molecular weight of the protein of. Superdex 75 increase belongs to the new generation of sec columns that replaces the wellknown superdex 75 columns. Safety for use and handling of the product in a safe way, refer to the safety data sheet. Superdex prep grade is a composite matr ix of dextran and.
Superdex 5150 gl and 10300 gl prepacked tricorn columns. Superdex prepacked columns are designed for highperformance, laboratoryscale separations of proteins, peptides, and other biomolecules according to size. Size exclusion chromatography columns and media selection guide. However, it remains the responsibility of the readers to familiarize themselves with the product information contained on the canadian product label or package insert. Selectivity curves from superdex 30 prep grade, superdex 75 prep grade, and. Every effort has been made to ensure the accuracy of the superdex 50% information published above. The steep selectivity of the dextran component and the high. Why the column pressure of superdex 75 10300 gl has increased to 1mpa even at a flow rate of 0. Ge healthcare size exclusion chromatography superdex 75. Superdex 75 increase belongs to the new generation of agarose based sec media, comprising smaller, more rigid beads with a narrower particle size distribution. Each is available in two column sizes, hiload 16600 and hiload 26600.
Superdex gel filtration media are listed in appendix c, table 1. Steep selectivity curves give gppd resolving power for peptides and proteins in the molecular weight range mr 10 000 600 000 superdex 200 pg. Selectivity curves from superdex 30 pg, superdex 75 pg and superdex 200 pg. Superdex 75 increase 5150 gl, superdex 200 increase 5150 gl and superose 6 increase 5150 gl are short columns with small bed volumes that are suitable. Superdex 75 increase cytiva, formerly ge healthcare life.
Superdex 75 increase is designed for smallscale preparative purification and analysis of recombinant tagged or other proteins with molecular weights from 3000 up to 70 000, such as recombinant tagged proteins. Superdex 30 increase is part of ges new generation of agarosebased sec columns called increase, which offer higher resolution purification in shorter runtimes compared with their predecessors. Short cycle time, together with small sample volume and low. The steep selectivity gives highresolution separations in the molecular weight range of 100 to 600 000. Separation of test substances on hiload 26600 superdex 30 pg. Equilibration of the column must be done with 2 bed volumes of the equilibration buffer before any experiment is. Ge healthcare superdex 75 increase prepacked columns are ideal when time is limited and low buffer and sample consumption is important. Superdex 30 prep grade, superdex 75 prep grade, and.
Quick information superdex 75 increase 5150 gl an d. Journal of chromatography, 537 1991 1732 elsevier science publishers b. We are using superdex increase 10300 gl size exclusion column in our lab and recently column pre column pressure is increased and flow rate is low around 0. The main characteristics of superdex media superdex. Rapid purity check and screening superdex 75 5150 gl, superdex 200 increase 5150 gl and superose 6 increase 5150 gl are short columns with small bed. The separation properties of these media are determined by the dextran componentchromatography columns separate molecules within mixtures during gas or liquid methods. Hiload 16600 and 26600 superdex 200 prep grade introduction hiload 16600 and 26600 superdex 30 prep grade, hiload 16600 and 26600 superdex 75 prep grade, and hiload 16600 and 26600 superdex 200 prep grade pg are prepacked xk columns designed for preparative gel filtration. Hiload superdex 75 pg columns m r 3 00070 000 hiload superdex 30 pg columns m r 1660 superdex 200 prep grade is a prepacked xk column for preparative gel filtration chromatography separations. Hiload superdex 75 prep grade data file hiload superdex 200. Calibration of hiload 2660 superdex 200 prep grade. Superdex prepacked columns fig 1 are designed for highperformance, laboratoryscale separations of proteins, peptides, and other biomolecules according to size.
Superdex prepacked columns are designed for highperformance, laboratoryscale separations of proteins, peptides, and other. Use columns prepacked with superdex 30 prep grade, superdex 75 prep grade. Rapid purity check and screening superdex 75 increase 5150 gl, superdex 200 increase 5150 gl and superose 6 increase 5150 gl are short columns with small bed volumes that are suitable for rapid protein homogeneity analyses or purity checks. Superdex prep grade is a matrix of dextran and highly crosslinked agarose. Superdex 30 prep grade medium is optimized for proteinspeptides below m r 10 000.
Ge healthcare lifescience superdex 200 increase 10300 gl prepacked tricorn columns are designed for small scale preparative purification. The columns are prepacked glass columns for high performance gel filtration of proteins, peptides, dna fragments superdex 75 increase 10300 gl is suitable for both smallscale preparative purification and for analytical applications. They save time when screening many samples, and require less buffer and sample than longer columns. Extracted from size exclusion chromatography principles and methods, pdf. Ge healthcare superdex 200 increase 10300 gl prepacked. Filled with stationary phase compositions or resins, the prepacked options reduce the preparing time required. Hiload 16600 and 26600 superdex 30 prep grade hiload 16. This leads to a collapse of the packed bed and the risk of poor. Protein purification chromatography calibration of. Hiload superdex 30 prep grade is optimized for proteins and peptides, but it can also be used with success to separate oligosaccharides. New superdex 200 increase columns product description ge cat. Superdex 75 10300 gl prepacked column for smallscale preparative purification g to mg or characterization of proteins in the molecular range 3000 to 70 000, like recombinant, tagged proteins. Superdex 75 10300 gl ge healthcare, 17517401, column l. Journal of chromatography, 547 1991 2 elsevier science publishers b.
Chemical, physical and chromatographic properties of. The steep selectivity of the dextran component 2 nonspecific interaction 3 column characteristics 4 operation 4 optimization 4 applications 5 hiload superdex 30 pg 5 hiload superdex 75 pg 6 hiload superdex 200 pg 6. Stability of superdex 75 prep grade and superdex 200 prep. Safety for use and handling of the products in a safe way, refer to the safety data sheet.
Ge healthcare superdex 75 increase prepacked column, 5150. Data file 18116379 ad gel filtration superdex highperformance columns fig 1. Our new generation columns called increase offer higher resolution and shorter run times than their predecessors. The columns are intended for use in preparative purification as well as for characterization and analysis of proteins with molecular weights between m r 3000 and 70 000, such as recombinant tagged proteins. Protein analysis with size exclusion chromatography sec. Data sources include ibm watson micromedex updated 10 apr 2020, cerner multum updated 6 apr 2020, wolters kluwer updated.
The high molecular weight kit contains 5 proteins with molecular weights in the range 44 000 to 669 000 and blue dextran 2000. Superdex 75 prep grade and superdex 200 prep grade are ideal for all stages. Hiload superdex columns, 200 pg, ge healthcare vwr. Why the column pressure of superdex 75 10300 gl has. Superdex 200 10300 gl chromatographic separation column. Highsample load 500 l separation of a mixture containing seven. Note that the whole fractionation range of superose 6 increase is not covered in this diagram.
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